Ultrasensitive C-peptide ELISA

(10-1141-01)

Kit format: 1×96 wells

Measurement range: 5-280 pmol/L (0.015-0.85 ng/mL)

1.High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L)

2.No or minimal cross-reactivity to insulin or proinsulin

3.High specificity enables measurement of human C-peptide in mouse and rat models

4.Calibrated against International Reference Reagent IRR84/510 for human C-peptide

5.CE/IVD labeled on select markets

6.Controls available: 10-1134-01 (US only) or 10-1164-01

DESCRIPTION

Mercodia Ultrasensitive C-peptide ELISA

A high-quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media, or urine. Even higher sensitivity can be achieved by using an alternative protocol described in technical note TN34-0144.

TEST CHARACTERISTICS

SPECIES：Human

SAMPLES：Serum, EDTA and heparin plasma or urine

SAMPLE VOLUME：50µL

ASSAY RANGE：5-280 pmol/L (0.015-0.85 ng/mL)

DETECTION LIMIT：≤2.5 pmol/L (0.0076 µg/L)

INCUBATION：1 h + 1 h + 30 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

INSULIN：< 0.0006 %

PROINSULIN：5 %

PROINSULIN (DES 31-32)：3 %

PROINSULIN (SPLIT 32-33)：2%

PROINSULIN (DES 64-65)：74%

PROINSULIN (SPLIT 65-66)：10%