Proinsulin ELISA

(10-1118-01)

Kit format: 1×96 wells

Measurement range:  3.3 - 130 pmol/L (0.03 - 1.18 µg/L)

1.Calibrated against International Reference Reagent IRR 84/611 for human proinsulin

2.Highly specific with no cross-reactivity to human insulin or C-peptide

3.Measures total proinsulin

4.Controls available: 10-1134-01 (US only) or 10-1164-01

DESCRIPTION

Mercodia Proinsulin ELISA provides a method for the quantitative determination of human proinsulin in serum or plasma.

TEST CHARACTERISTICS

SPECIES：Human

SAMPLES：Serum, plasma

SAMPLE VOLUME：50µL

ASSAY RANGE：3.3 - 130 pmol/L (0.03 - 1.18 µg/L)

DETECTION LIMIT：≤1.7 pmol/L

INCUBATION：1 h + 1 h + 15 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

INSULIN：< 0.03 %

C-PEPTIDE：< 0.006 %

PROINSULIN DES (31-32)：95 %

PROINSULIN SPLIT (32-33)：95 %

PROINSULIN DES (64-65)：84%

PROINSULIN SPLIT (65-66)：90 %

PORCINE PROINSULIN：4%

MOUSE PROINSULIN I：3%

MOUSE PROINSULIN II：16%

RAT PROINSULIN I：13%

RAT PROINSULIN II：10 %